Sexing bovine embryos using PCR amplification of bovine SRY sequence

Y T Zeng; M L Zhang; M J Chen; X D Zhou; Y Huang; Z R Ren; S Z Huang; M X Hu; X Q Wu; J M Gao

Sexing bovine embryos using PCR amplification of bovine SRY sequence

Sci China B. 1994 Feb;37(2):170-6.

Authors

Y T Zeng¹, M L Zhang, M J Chen, X D Zhou, Y Huang, Z R Ren, S Z Huang, M X Hu, X Q Wu, J M Gao, et al.

Affiliation

¹ Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, PRC.

PMID: 8068189

Abstract

This study analyses the bovine SRY DNA sequence by direct sequencing procedure, followed by the designation of the PCR primers specific for bovine SRY. Using PCR amplification of bovine SRY gene, the embryo sex was determined. The results of the embryo sex identification were confirmed after the embryo transfer and pregnancies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cattle
DNA / genetics*
Embryo Transfer
Female
Male
Polymerase Chain Reaction
Sex Determination Analysis*
Y Chromosome*

Substances

DNA