Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions

J Clin Microbiol. 1994 May;32(5):1349-53. doi: 10.1128/jcm.32.5.1349-1353.1994.

Abstract

A monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed for specific detection of peste des petits ruminants virus. Compared with virus isolation in Vero cell cultures using 89 paired tissue and secretion samples from six experimentally infected goats, S-ELISA was significantly more sensitive (71.9% versus 65.2%; P < 0.05). The S-ELISA is a suitable alternative to virus isolation.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Viral / isolation & purification
  • Diagnosis, Differential
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / statistics & numerical data
  • Evaluation Studies as Topic
  • Goat Diseases / diagnosis*
  • Goat Diseases / microbiology
  • Goats
  • Morbillivirus Infections / diagnosis
  • Morbillivirus Infections / microbiology
  • Morbillivirus Infections / veterinary*
  • Peste-des-petits-ruminants virus / immunology*
  • Peste-des-petits-ruminants virus / isolation & purification*
  • Rinderpest / diagnosis
  • Sensitivity and Specificity
  • Vero Cells
  • Virology / methods*

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral