Isolation of mouse complement component C7

Y Hitsumoto; H Ohnishi; M Okada; H Nishimukai; S Saheki; N Takeuchi

doi:10.1016/0022-1759(94)90310-7

Isolation of mouse complement component C7

J Immunol Methods. 1994 Dec 2;176(2):163-7. doi: 10.1016/0022-1759(94)90310-7.

Authors

Y Hitsumoto¹, H Ohnishi, M Okada, H Nishimukai, S Saheki, N Takeuchi

Affiliation

¹ Department of Clinical Laboratory Medicine, Ehime University School of Medicine, Japan.

PMID: 7983377
DOI: 10.1016/0022-1759(94)90310-7

Abstract

Mouse complement component C7 was purified from serum by a sequential procedure of fractionation precipitation by ammonium sulfate, followed by DE-52 anion exchange chromatography. Protein G affinity column chromatography, Mono S cation exchange chromatography and Superdex 200 gel filtration. The final product contained a highly purified mouse C7 component showing a single band on SDS-PAGE at the apparent Mrs of 90 kDa and 100 kDa under non-reduced and reduced conditions respectively. The yield of C7, which was measured by the biological activity, was 7.0%

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chemical Fractionation
Chromatography / methods
Complement C7 / analysis
Complement C7 / isolation & purification*
Electrophoresis, Polyacrylamide Gel
Mice
Mice, Inbred DBA

Substances

Complement C7