Antiviral activity of primate gamma delta T lymphocytes isolated by magnetic cell sorting

J Med Primatol. 1994 Feb-May;23(2-3):131-5. doi: 10.1111/j.1600-0684.1994.tb00113.x.

Abstract

The exposure of human or rhesus monkey peripheral blood mononuclear cells (PBMCs) to interleukin 2 (IL-2) in vitro resulted in a selective outgrowth of gamma delta lymphocytes. Using positive selection by monoclonal antibodies and magnetic beads, gamma delta T lymphocytes were isolated from these cultures. Without priming by viral antigens, the purified gamma delta T lymphocytes lyse immunodeficiency virus-infected cells substantially better than the uninfected counterparts.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Cells, Cultured
  • Cytotoxicity, Immunologic
  • Flow Cytometry
  • HIV / immunology
  • HIV / pathogenicity*
  • Humans
  • Immunomagnetic Separation
  • Interleukin-2 / pharmacology*
  • Macaca mulatta
  • Receptors, Antigen, T-Cell, gamma-delta / immunology*
  • Reference Values
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Tumor Cells, Cultured

Substances

  • Interleukin-2
  • Receptors, Antigen, T-Cell, gamma-delta