Abstract
Screening a small cell lung cancer cDNA library with serum from a patient with antibody-associated paraneoplastic sensory neuronopathy (Anti-Hu syndrome) resulted in the isolation of a cDNA clone encoding the Ro-52 kD antigen. The Ro-52 kD antigen is one of the major antigens recognized by the sera of patients with Sjögren's syndrome and systemic lupus erythematosus. Further investigation revealed that the sera of only a small percentage (4%) of patients with the Hu syndrome reacted with Ro-52 kD antigen. The cross-reactivity may result from a domain of high homology between Ro-52 kD and HuD antigen. These results emphasize the necessity of using recombinant antigens whenever possible when assaying for assaying for specific antibodies.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antibodies / immunology*
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Autoantigens / genetics
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Autoantigens / immunology*
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Base Sequence
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Blood / immunology*
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Cloning, Molecular
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DNA, Complementary / genetics
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ELAV Proteins
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ELAV-Like Protein 4
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Encephalomyelitis / blood*
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Humans
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Molecular Probes / genetics
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Molecular Sequence Data
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Molecular Weight
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Nerve Tissue Proteins*
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Paraneoplastic Syndromes / blood*
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RNA, Small Cytoplasmic*
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RNA-Binding Proteins / immunology
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Recombinant Fusion Proteins / immunology
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Ribonucleoproteins / genetics
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Ribonucleoproteins / immunology*
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Sensation Disorders / blood*
Substances
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Antibodies
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Autoantigens
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DNA, Complementary
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ELAV Proteins
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ELAV-Like Protein 4
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ELAVL4 protein, human
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Molecular Probes
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Nerve Tissue Proteins
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RNA, Small Cytoplasmic
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RNA-Binding Proteins
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RO60 protein, human
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Recombinant Fusion Proteins
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Ribonucleoproteins
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SS-A antigen