The prevalence of Sarcocystis infection was examined in older cows and imported cattle slaughtered in East Hokkaido. Samples of myocardial tissues were examined for Sarcocystis microscopically. Sarcocystis cysts were detected in 15.7% of 83 older cows, 48.4% of 91 imported cattle which were kept in East Hokkaido prior to slaughter and 51.1% of 94 imported cattle slaughtered immediately after quarantine check. Based on the morphology of the cyst wall and the establishment of infection in experimentally inoculated dog, the Sarcocystis species was identified as Sarcocystis cruzi.