Abstract
Embryonic stem cells (ES) can be exploited to analyze in vitro mechanisms of cellular differentiation. We have utilized ES-derived embryoid body formation in an attempt to study cell types resulting from in vitro differentiation. To this end, a variety of molecular markers, preferably those which have been associated with regulatory events during mouse embryogenesis, was employed. Specifically, Brachyury (T), Pax-3 and Pax-6 genes as well as Nkx-1.1 were used. We could demonstrate that the expression of these genes in vitro was regulated by growth factors such as activin A or bFGF. Implications of these findings and the possible applications for identifying new genes are discussed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Brachyury Protein
-
Cells, Cultured
-
DNA-Binding Proteins / biosynthesis*
-
Embryo, Nonmammalian
-
Embryonic and Fetal Development
-
Eye Proteins
-
Fetal Proteins / biosynthesis*
-
Gene Expression Regulation* / drug effects
-
Genes, Homeobox*
-
Glial Fibrillary Acidic Protein / analysis
-
Homeodomain Proteins*
-
In Situ Hybridization, Fluorescence
-
Mesoderm / drug effects
-
Mesoderm / physiology
-
Nerve Growth Factors / pharmacology*
-
Neurons / cytology
-
Neurons / physiology
-
PAX3 Transcription Factor
-
PAX6 Transcription Factor
-
Paired Box Transcription Factors
-
Repressor Proteins
-
Stem Cells / drug effects
-
Stem Cells / metabolism*
-
T-Box Domain Proteins*
-
Transcription Factors*
-
Xenopus
-
Xenopus Proteins*
Substances
-
DNA-Binding Proteins
-
Eye Proteins
-
Fetal Proteins
-
Glial Fibrillary Acidic Protein
-
Homeodomain Proteins
-
Nerve Growth Factors
-
PAX3 Transcription Factor
-
PAX6 Transcription Factor
-
Paired Box Transcription Factors
-
Pax3 protein, Xenopus
-
Pax6 protein, mouse
-
Repressor Proteins
-
T-Box Domain Proteins
-
Transcription Factors
-
Xenopus Proteins
-
Pax3 protein, mouse
-
Brachyury Protein