Molecular cloning and polymerase chain reaction-sequence-specific oligonucleotide detection of the allele encoding the novel allospecificity HLA-Cw6.2 (Cw*1502) in Spanish gypsies

Hum Immunol. 1993 Aug;37(4):259-63. doi: 10.1016/0198-8859(93)90509-y.

Abstract

A proposed novel allospecificity, HLA-Cw6.2, has been reported to be commonly found (approximately 25%) in Spanish Gypsies. Full-length cDNAs of the allele (Cw*1502) coding for this antigen have been cloned in this study. A simple PCR-SSO method for its detection has been standardized and a correlation with the serologic Cw6.2 phenotype has been established. This specificity has been also detected in the homozygous typing cell RML. Although the primary structures of Cw*1502 and Cw*0601 are not closely related, they share specific motifs that may account for their serologic cross-reactivity. Two novel HLA-C alleles (Cw*12022 and Cw*0602) are also reported.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular*
  • Consensus Sequence
  • DNA / analysis
  • HLA-C Antigens / genetics*
  • Histocompatibility Testing
  • Humans
  • Immunophenotyping
  • Molecular Sequence Data
  • Oligonucleotides / genetics*
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • Protein Structure, Tertiary
  • Roma / genetics*
  • Spain

Substances

  • HLA-C Antigens
  • HLA-C*06 antigen
  • Oligonucleotides
  • DNA

Associated data

  • GENBANK/X67818
  • GENBANK/X70856
  • GENBANK/X70857