Transportation of Helicobacter pylori cultures by optimal systems

J Clin Microbiol. 1994 Dec;32(12):3075-7. doi: 10.1128/jcm.32.12.3075-3077.1994.

Abstract

Cultures of Helicobacter pylori on chocolate agar slants in bijou bottles and on chocolate agar plates inside BBL Campy Pouches were mailed from Dublin to Galway, Ireland; Bordeaux, France; and Beijing, China. Both systems maintained viability of H. pylori for at least 4 days under mailing conditions. Ninety percent of the isolates on the slants survived for 6 days, but only 30% of the isolates in the pouches survived. When the slants were stored at 4 degrees C after arrival, 50% of the isolates were recoverable 10 days after mailing. Failure of recovery was due to coccoid formation by the organisms. Contamination was not a problem in either system. Chocolate agar slants are considered the more suitable system for transporting H. pylori cultures, especially when transport time longer than 4 days is expected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agar
  • Bacteriological Techniques*
  • Culture Media
  • Helicobacter pylori*
  • Humans
  • Specimen Handling*
  • Time Factors
  • Transportation*

Substances

  • Culture Media
  • Agar