Quantitative analysis of DNA aberrations amplified by competitive polymerase chain reaction using capillary electrophoresis

J Chromatogr B Biomed Appl. 1994 Oct 14;660(2):271-7. doi: 10.1016/0378-4347(94)00285-1.

Abstract

We compared the use of capillary electrophoresis (CE) in a polymer network with the use of slab gel electrophoresis for the quantitative analysis of polymerase chain reaction (PCR)-amplified DNA samples. We quantified residual lymphoma cells carrying a translocation between chromosomes 14 and 18, in consecutive patient peripheral blood samples that were amplified by competitive PCR. For CE analysis we used a 4% linear polyacrylamide network. Results show that the calculated number of translocations in patient samples using both analyses were comparable. We conclude that CE is a sensitive, non-radioactive, fast and accurate method for quantitation of competitive PCR products.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography
  • Base Sequence
  • Chromosomes, Human, Pair 14
  • Chromosomes, Human, Pair 18
  • DNA / analysis*
  • Electrophoresis
  • Humans
  • Lymph Nodes / chemistry
  • Lymphoma / chemistry
  • Molecular Sequence Data
  • Oligonucleotides / analysis
  • Polymerase Chain Reaction
  • Translocation, Genetic

Substances

  • Oligonucleotides
  • DNA