Atypical beta-adrenoceptor in bovine adrenal medulla

Eur J Pharmacol. 1994 Oct 3;263(3):R1-2. doi: 10.1016/0014-2999(94)90730-7.

Abstract

Bovine adrenal medullary membranes were incubated with [125I]cyanopindolol to assess beta-adrenoceptor binding. Binding was saturable and specific; a single low affinity site (Kd = 750 pM) was identified. [125I]Cyanopindolol binding was displaced by micromolar concentrations of classic beta-adrenoceptor antagonists and by sodium-4-[-2-[2-hydroxy-2-(-3-chloro-phenyl) ethylamino] propyl] phenoxyacetate. These data are similar to reported binding of beta 3-adrenoceptors and may explain beta-adrenoceptor agonist modulation of chromaffin cell degranulation in this catecholamine rich environment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Medulla / cytology
  • Adrenal Medulla / drug effects
  • Adrenal Medulla / metabolism*
  • Adrenergic beta-Agonists / metabolism
  • Adrenergic beta-Agonists / pharmacology*
  • Adrenergic beta-Antagonists / metabolism
  • Adrenergic beta-Antagonists / pharmacology*
  • Animals
  • Binding, Competitive
  • Cattle
  • Cell Degranulation / drug effects
  • Pindolol / analogs & derivatives
  • Pindolol / metabolism
  • Pindolol / pharmacology
  • Radioligand Assay
  • Receptors, Adrenergic, beta / drug effects
  • Receptors, Adrenergic, beta / metabolism*

Substances

  • Adrenergic beta-Agonists
  • Adrenergic beta-Antagonists
  • Receptors, Adrenergic, beta
  • cyanopindolol
  • Pindolol