Lack of correlation between mRNA expression and enzymatic activity of the aspartate aminotransferase isoenzymes in various tissues of the rat

FEBS Lett. 1995 Jun 12;366(2-3):170-2. doi: 10.1016/0014-5793(95)00518-e.

Abstract

Little is known about control of expression of basal levels of the aspartate aminotransferases which are ubiquitous 'house keeping' enzymes in vertebrates. We have measured both mRNA and activity levels for both isoenzymes in various rat tissues as a function of age. Patterns of mRNA expression for the two isoenzymes were similar in a particular tissue about differed widely between tissues. Surprisingly, there was no simple correlation between mRNA levels and specific activities of the enzyme products. We conclude that translation for mRNA for these two isoenzymes is subject to tissue-specific, and in some cases age-related, regulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Aspartate Aminotransferases / genetics*
  • Aspartate Aminotransferases / metabolism
  • Enzyme Induction
  • Isoenzymes / genetics*
  • Isoenzymes / metabolism
  • Male
  • Mitochondria / enzymology
  • Muscle Proteins / genetics
  • Muscle Proteins / metabolism
  • Organ Specificity
  • Protein Biosynthesis
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar

Substances

  • Isoenzymes
  • Muscle Proteins
  • RNA, Messenger
  • Aspartate Aminotransferases