Abstract
Staphylococcal nuclease (SNase), a 14 kD enzyme that catalyzes the hydrolysis of single- and double-stranded nucleic acid, was fused to the N-terminus of the gene III (pIII) protein of filamentous phage fdtet. The SNase-pIII protein is infective and the catalyzes DNA hydrolysis, demonstrating that functional SNase can be displayed on the phage surface.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacteriophages / enzymology
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Bacteriophages / genetics*
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Base Sequence
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Blotting, Western
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Capsid / chemistry
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Capsid / genetics
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Capsid / metabolism
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Cloning, Molecular
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Genetic Vectors
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Micrococcal Nuclease / genetics
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Micrococcal Nuclease / metabolism*
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Molecular Sequence Data
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transfection / genetics
Substances
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Recombinant Fusion Proteins
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Micrococcal Nuclease