Sequencing of heparan sulfate proteoglycans: identification of variable and constant oligosaccharide regions in eight heparan sulfate proteoglycans of different origins

Braz J Med Biol Res. 1994 Sep;27(9):2097-102.

Abstract

The sequence of the disaccharide units of eight heparan sulfate proteoglycans of different origins is described. All heparan sulfates contain 5 variable regions made of oligosaccharide blocks of disaccharides, namely, GlcUA(1-4)GlcNAc, GlcUA(1-4)GlcNS, IdoUA (1-4) GlcNS,6S,IdoUA-GlcNAc,6S, and IdoUA,2S(1-4)GlcNS,6S, besides two constant regions made of an internal tetrasaccharide (IdoUA-GlcNAc-IdoUA-GlcNS) and monosaccharides (GlcNS, and GlcNS,6S) at the non-reducing terminal. The N-acetylated region of the heparan sulfates is linked to the serine of the protein core through a trisaccharide of Xyl-Gal-Gal. Heparan sulfates differ from one another in terms of the number of disaccharides that compose each block.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Carbohydrate Sequence
  • Cattle
  • Chemical Fractionation
  • Disaccharides / chemistry
  • Dogs
  • Heparan Sulfate Proteoglycans
  • Heparitin Sulfate / chemistry*
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Polysaccharide-Lyases / analysis
  • Proteoglycans / chemistry*
  • Rabbits
  • Sequence Analysis

Substances

  • Disaccharides
  • Heparan Sulfate Proteoglycans
  • Oligosaccharides
  • Proteoglycans
  • Heparitin Sulfate
  • Polysaccharide-Lyases
  • heparitinsulfate lyase