[Interleukin-1 beta, interleukin-1 receptor antagonist mRNA expression of peripheral blood mononuclear cells (PBMC) in idiopathic nephrotic syndrome (INS) detected by biotin-labelled probe in situ hybridization]

Zhonghua Yi Xue Za Zhi. 1995 Mar;75(3):152-4, 190.
[Article in Chinese]

Abstract

The pathogenesis of INS is related to the disorder of cellular immunity. Using the in situ hybridization of biotin-labeled probe and streptavidin -alkaline phosphatase conjugate detection system, we quantitatively analysed by true colour medical image analysis system the expression of IL-1 beta, IL-1ra mRNA of peripheral blood mononuclear cells (PBMC) from 4 cases of idiopathic nephrotic syndrome. IL-1 beta IL-1ra mRNA expression of PBMC in INS were significantly lower than those of the normal control (107.63 +/- 20.80 vs 195.56 +/- 31.14; 144.45 +/- 11.42 vs 370.38 +/- 100.48, respectively, P < 0.05). The expression of IL-1 beta mRNA was significantly lower than the IL-1ra mRNA expression of PBMC in INS (107.63 +/- 20.80 vs 144.45 +/- 11.42, P < 0.05). There existed dysregulation of IL-1 beta, IL-1ra mRNA expression in INS. The advantages of biotin-labelled probe in situ hybridization were also discussed.

Publication types

  • English Abstract

MeSH terms

  • Child
  • Female
  • Gene Expression
  • Humans
  • In Situ Hybridization
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / genetics*
  • Interleukin-1beta
  • Leukocytes, Mononuclear / immunology*
  • Male
  • Nephrotic Syndrome / blood*
  • Peptide Fragments / genetics*
  • RNA, Messenger / metabolism*
  • Receptors, Interleukin-1 / antagonists & inhibitors
  • Receptors, Interleukin-1 / genetics*
  • Sialoglycoproteins / genetics*

Substances

  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Interleukin-1beta
  • Peptide Fragments
  • RNA, Messenger
  • Receptors, Interleukin-1
  • Sialoglycoproteins
  • interleukin-1beta (163-171)