Role of excitatory amino-acids in diethyldithiocarbamate-induced cell death in mesencephalic cultures

Brain Res. 1995 Mar 13;674(1):127-32. doi: 10.1016/0006-8993(94)01457-s.

Abstract

The effects of diethyldithiocarbamate (DDC) and DDC plus glutamate on mesencephalic cell cultures were investigated. DDC 10 microM was toxic for cell cultures as assessed by observation under a phase-contrast microscope and the drop in [3H]dopamine uptake. Moreover, DDC 1 microM greatly potentiated cell death induced by glutamate 10 and 50 microM. (+)MK801, a selective non-competitive antagonist of NMDA receptors, completely prevented the toxicity of the two neurotoxins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death / drug effects
  • Cells, Cultured
  • Ditiocarb / poisoning*
  • Dizocilpine Maleate / pharmacology
  • Drug Synergism
  • Excitatory Amino Acids / physiology*
  • Glutamic Acid / poisoning
  • Mesencephalon / cytology
  • Mesencephalon / drug effects*
  • Mice
  • Neurons / drug effects*
  • Neuroprotective Agents / pharmacology

Substances

  • Excitatory Amino Acids
  • Neuroprotective Agents
  • Glutamic Acid
  • Dizocilpine Maleate
  • Ditiocarb