A novel method for transfer of two-dimensional crystals from the air/water interface to specimen grids. EM sample preparation/lipid-layer crystallization

J Struct Biol. 1995 Jan-Feb;114(1):60-6. doi: 10.1006/jsbi.1995.1005.

Abstract

Transfer of two-dimensional (2-D) crystals formed on lipid layers by suspension from a wire loop is described. This method gives better recovery and better preservation of 2-D crystals than attained in the past. The method has been applied to crystals of yeast RNA polymerase II to enable their analysis in the frozen hydrated state.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Air
  • Carbon
  • Crystallization*
  • Fungal Proteins
  • Lipids
  • Microscopy, Electron / instrumentation
  • Microscopy, Electron / methods*
  • Proteins*
  • RNA Polymerase II
  • Reproducibility of Results
  • Saccharomyces cerevisiae / enzymology
  • Specimen Handling / methods*
  • Water

Substances

  • Fungal Proteins
  • Lipids
  • Proteins
  • Water
  • Carbon
  • RNA Polymerase II