Molecular structure and function of angiotensin type 2 receptor

Kidney Int. 1994 Dec;46(6):1502-4. doi: 10.1038/ki.1994.430.

Abstract

More than two isoforms have been identified for angiotensin receptors based on their ligand selectivity. The objective of this study is to determine the molecular structure of angiotensin type 2 receptor (AT2), whose physiological functions are still an enigma despite extensive studies on its distribution in fetal tissues. We expression-cloned a cDNA of an affinity-purified AT2 from rat pheochromocytoma cells (PC12w). The AT2 cDNA clone comprises 2,868 nucleotides and encodes a 363 amino acid protein with seven putative transmembrane domains. The dissociation constant for its binding to 125I-CGP42112A, an AT2-specific ligand, was 0.11 +/- 0.01 nM. Its binding to 0.5 nM 125I-[Sar1,Ile8]-Ang II was not inhibited by Dup 753 but by PD123319 (IC50 = 1.7 +/- 0.2 nM). These binding features are characteristic of angiotensin type 2 receptor. The amino acid sequence analysis of the purified AT2 corroborated the amino terminus of the deduced primary structure of AT2. Angiotensin type 1 receptor (AT1) is the most closely related to AT2 but with only 32% amino acid sequence identity. Angiotensin II attenuated membrane-associated protein tyrosine phosphatase activity in the COS-7 cells stably expressing AT2 through a pertussis toxin-sensitive G protein. However, the physiological function of AT2 in the fetal kidney is still unresolved.

MeSH terms

  • Amino Acid Sequence
  • Angiotensin Receptor Antagonists
  • Animals
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Kidney / physiology
  • Kinetics
  • Molecular Sequence Data
  • Molecular Structure
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Tyrosine Phosphatases / metabolism
  • Rats
  • Receptors, Angiotensin / genetics*
  • Receptors, Angiotensin / physiology
  • Sequence Homology, Amino Acid
  • Structure-Activity Relationship
  • Transfection

Substances

  • Angiotensin Receptor Antagonists
  • DNA, Complementary
  • Receptors, Angiotensin
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Tyrosine Phosphatases