Changes in ether-linked phospholipids in rat kidney by dietary alpha-linolenic acid in vivo

Lipids. 1995 Jun;30(6):541-6. doi: 10.1007/BF02537029.

Abstract

We investigated the effects of perilla oil containing a high level of alpha-linolenic acid on in vivo phospholipid metabolism, particularly three subclasses of choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP), in rat kidney. After three weeks of feeding, a significantly lower proportion (by 35%) of the alkylacyl subclass of CGP was found in the perilla oil, as compared to corn oil-fed animals. The alkylacyl species of EGP was also higher in the perilla oil than in the corn oil-fed animals. These alterations were accompanied by a remarkably lower proportion of arachidonic acid and a higher level of eicosapentaenoic acid (EPA) in all six subclasses of CGP and EGP in the perilla oil-fed animals. The levels of linoleic acid were even higher in the diacyl subclasses of CGP and EGP in the perilla oil group, suggesting that desaturase and elongase enzymes prefer n-3 to n-6 fatty acids as substrates for diacyl species. These data are useful in defining the effects of alpha-linolenic acid on the biosynthesis of renal phospholipids and on the replacement of n-6 with n-3 fatty acids in the six CGP and EGP subclasses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism
  • Corn Oil / administration & dosage
  • Dietary Fats, Unsaturated / administration & dosage
  • Dietary Fats, Unsaturated / pharmacology*
  • Eicosapentaenoic Acid / metabolism
  • Ethers / metabolism*
  • Female
  • Kidney / drug effects
  • Kidney / metabolism*
  • Phosphatidylcholines / metabolism
  • Phosphatidylethanolamines / metabolism
  • Phospholipids / metabolism*
  • Rats
  • Rats, Inbred F344
  • alpha-Linolenic Acid / administration & dosage
  • alpha-Linolenic Acid / pharmacology*

Substances

  • Dietary Fats, Unsaturated
  • Ethers
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Phospholipids
  • alpha-Linolenic Acid
  • Arachidonic Acid
  • Corn Oil
  • Eicosapentaenoic Acid