Inhibitory effect of probucol on nephrotoxicity induced by ferric nitrilotriacetate (Fe-NTA) in rats

Carcinogenesis. 1995 Oct;16(10):2549-52. doi: 10.1093/carcin/16.10.2549.

Abstract

Effects of dietary probucol on renal damage induced by a renal carcinogen, ferric nitrilotriacetate (Fe-NTA), in male Wistar rats were quantitatively investigated with a computer-mediated image analyzer. The kidneys of rats fed a 1% probucol diet were protected from necrosis and lipid peroxidation induced by a single i.p. treatment with Fe-NTA solution at 5 mg Fe/kg body wt and were significantly resistant to a higher dose. For the parameter of lipid peroxidation, Schiff's staining method was utilized to demonstrate the extent of formation of aldehydes, products of lipid peroxidation. Thus following injection of Fe-NTA solution at 10 mg Fe/kg body wt the average areas of tubular necrosis were 85.8% versus 56.9% and the positive areas for Schiff's staining were 15.3% versus 5.6% in the renal cortices of rats fed control of 1% probucol diets respectively. These results indicate that probucol provides protection against Fe-NTA-induced nephrotoxicity through its antioxidant properties. In addition to being a cholesterol-lowering drug, useful for the control of hypercholesterolemia, probucol may therefore be beneficial for prevention and treatment of various pathogenic processes including those leading to carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticarcinogenic Agents / administration & dosage
  • Anticarcinogenic Agents / pharmacology*
  • Carcinogens / toxicity*
  • Diet
  • Ferric Compounds / toxicity*
  • Kidney / drug effects
  • Kidney / pathology*
  • Kidney Cortex / drug effects
  • Kidney Cortex / pathology
  • Kidney Neoplasms / chemically induced
  • Kidney Neoplasms / pathology
  • Kidney Neoplasms / prevention & control*
  • Male
  • Nitrilotriacetic Acid / analogs & derivatives*
  • Nitrilotriacetic Acid / toxicity
  • Probucol / administration & dosage
  • Probucol / pharmacology*
  • Rats
  • Rats, Wistar

Substances

  • Anticarcinogenic Agents
  • Carcinogens
  • Ferric Compounds
  • Nitrilotriacetic Acid
  • Probucol
  • ferric nitrilotriacetate