Age-associated change of 8-hydroxyguanine repair activity in cultured human fibroblasts

Biochem Biophys Res Commun. 1995 Sep 25;214(3):1157-62. doi: 10.1006/bbrc.1995.2407.

Abstract

8-hydroxyguanine (8-OH-Gua) is a major oxidative DNA damage product. Its repair activity was determined in the human fibroblast cell line, TIG-3, during the aging process. The repair activity increased in the first part of the aging process and decreased in the latter part. The highest repair activity was observed at the age of 30 PDL. These results suggest that the defense capacity for replication errors due to oxidative DNA damage increases greatly when the cells are rapidly dividing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • Cellular Senescence
  • DNA Damage*
  • DNA Repair*
  • Endonucleases / metabolism
  • Fetus
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibroblasts / physiology
  • Guanine / analogs & derivatives*
  • Guanine / metabolism
  • Humans
  • Kinetics
  • Male
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemical synthesis
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / metabolism
  • Oxidative Stress
  • Time Factors

Substances

  • Oligodeoxyribonucleotides
  • 8-hydroxyguanine
  • Guanine
  • Endonucleases