Diversity among Leishmania isolates from the Sudan: isoenzyme homogeneity of L. donovani versus heterogeneity of L. major

M E Ibrahim; D A Evans; T G Theander; A M el Hassan; A Kharazmi

doi:10.1016/0035-9203(95)90010-1

Diversity among Leishmania isolates from the Sudan: isoenzyme homogeneity of L. donovani versus heterogeneity of L. major

Trans R Soc Trop Med Hyg. 1995 Jul-Aug;89(4):366-9. doi: 10.1016/0035-9203(95)90010-1.

Authors

M E Ibrahim¹, D A Evans, T G Theander, A M el Hassan, A Kharazmi

Affiliation

¹ Centre for Medical Parasitology, University of Copenhagen, Denmark.

PMID: 7570863
DOI: 10.1016/0035-9203(95)90010-1

Abstract

Leishmania isolates from patients in the Sudan suffering from either visceral or cutaneous leishmaniasis were characterized using a battery of 12 enzymes. Aspartate aminotransferase separated the L. donovani isolates into 2 distinct zymodemes, but the overall results showed no significant geographical variation among L. donovani isolates. In contrast, the isolates of L. major were polymorphic, exhibiting differences in nucleoside hydrolase, 6-phosphogluconate dehydrogenase, superoxide dismutase, esterase, mannose phosphate isomerase, and aspartate aminotransferase, resulting in the description of 4 new enzymatic variants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Isoenzymes / isolation & purification*
Leishmania donovani / enzymology*
Leishmania major / enzymology*
Leishmaniasis, Cutaneous / enzymology
Leishmaniasis, Cutaneous / parasitology*
Leishmaniasis, Visceral / enzymology
Leishmaniasis, Visceral / parasitology*
Sudan

Substances

Isoenzymes