Trans-activation of the wheat histone H3 promoter by Gal4 DNA-binding domain (1-94) in plant cells

Biochim Biophys Acta. 1995 Sep 19;1263(3):281-4. doi: 10.1016/0167-4781(95)00117-y.

Abstract

This is the first demonstration that Gal4 DNA-binding domain (1-94) itself has the ability to trans-activate an eukaryotic promoter in vivo. It significantly activated a wheat histone H3 core promoter, but slightly activated a CaMV 35S core promoter in transiently transfected tobacco cells when the Gal4-recognition sequences were put upstream of the core promoters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • DNA-Binding Proteins / genetics
  • Genes, Reporter
  • Histones / genetics*
  • Molecular Sequence Data
  • Nicotiana / genetics
  • Plants, Toxic
  • Promoter Regions, Genetic*
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors / genetics*
  • Transcriptional Activation
  • Triticum / genetics*

Substances

  • DNA-Binding Proteins
  • GAL4 (1-147) protein, S cerevisiae
  • Histones
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors