Molecular cloning and sequencing of the gene encoding a sheep arginine vasopressin type 1a receptor

A M Hutchins; P A Phillips; D J Venter; L M Burrell; C I Johnston

doi:10.1016/0167-4781(95)00125-z

Molecular cloning and sequencing of the gene encoding a sheep arginine vasopressin type 1a receptor

Biochim Biophys Acta. 1995 Sep 19;1263(3):266-70. doi: 10.1016/0167-4781(95)00125-z.

Authors

A M Hutchins¹, P A Phillips, D J Venter, L M Burrell, C I Johnston

Affiliation

¹ Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.

PMID: 7548217
DOI: 10.1016/0167-4781(95)00125-z

Abstract

The gene for the sheep arginine vasopressin type 1a (V1a) receptor subtype was cloned from a genomic library. The deduced amino acid sequence shows characteristics of a G-protein coupled receptor and high sequence identity to human and rat V1a receptor sequences (81% and 73%, respectively). Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed a tissue distribution consistent with a type V1a receptor. The genomic DNA (7.1 kb) contains a 1586 bp intron between the putative 6th and 7th transmembrane domains.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA / chemistry
Genomic Library
Molecular Sequence Data
Receptors, Vasopressin / genetics*
Sequence Alignment
Sequence Homology, Amino Acid
Sheep

Substances

Receptors, Vasopressin
DNA

Associated data

GENBANK/L41502