Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector

E Kinner; D Pocta; S Ströer; H Schmieger

doi:10.1111/j.1574-6968.1994.tb06842.x

Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector

FEMS Microbiol Lett. 1994 May 15;118(3):283-9. doi: 10.1111/j.1574-6968.1994.tb06842.x.

Authors

E Kinner¹, D Pocta, S Ströer, H Schmieger

Affiliation

¹ Institut für Genetik und Mikrobiologie, Universität München, FRG.

PMID: 7545934
DOI: 10.1111/j.1574-6968.1994.tb06842.x

Abstract

The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram-positive bacteria that have been studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophages / genetics*
Cloning, Molecular
Cosmids / genetics*
Genetic Vectors / genetics*
Genome, Viral
Restriction Mapping
Sequence Analysis, DNA
Streptomyces*
Transduction, Genetic*

Associated data

GENBANK/X65555