Localization of 12-lipoxygenase mRNA in cultured oligodendrocytes and astrocytes by in situ reverse transcriptase and polymerase chain reaction

M K Bendani; O Palluy; J Cook-Moreau; J L Beneytout; M Rigaud; J M Vallat

doi:10.1016/0304-3940(95)11482-c

Localization of 12-lipoxygenase mRNA in cultured oligodendrocytes and astrocytes by in situ reverse transcriptase and polymerase chain reaction

Neurosci Lett. 1995 Apr 21;189(3):159-62. doi: 10.1016/0304-3940(95)11482-c.

Authors

M K Bendani¹, O Palluy, J Cook-Moreau, J L Beneytout, M Rigaud, J M Vallat

Affiliation

¹ URA-CNRS 1485, Faculté de Médecine, Limoges, France.

PMID: 7542757
DOI: 10.1016/0304-3940(95)11482-c

Abstract

We studied the distribution of 12-lipoxygenase mRNA in glial cells. First, mRNA was detected from cellular extracts by soluble-phase reverse transcriptase-polymerase chain reaction (RT-PCR). Taking into account that cell culture populations could not be 100% homogeneous, we then developed, for the first time, an in situ RT-PCR combined with immunocytochemistry with cell specific markers. Using this procedure we showed that 12-lipoxygenase mRNA was expressed both in mature oligodendrocytes and astrocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Newborn
Arachidonate 12-Lipoxygenase / biosynthesis*
Astrocytes / enzymology*
Base Sequence
Cells, Cultured
Immunohistochemistry
Molecular Sequence Data
Oligodendroglia / enzymology*
Polymerase Chain Reaction
RNA, Messenger / biosynthesis*
RNA-Directed DNA Polymerase
Rats
Tissue Fixation

Substances

RNA, Messenger
Arachidonate 12-Lipoxygenase
RNA-Directed DNA Polymerase