Abstract
Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Line
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Evoked Potentials
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Green Fluorescent Proteins
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Humans
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Ion Channel Gating
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Ion Channels / biosynthesis*
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Ion Channels / physiology
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Kidney
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Luminescent Proteins / analysis
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Luminescent Proteins / biosynthesis*
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Macromolecular Substances
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Microscopy, Fluorescence / methods
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Patch-Clamp Techniques
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Polymerase Chain Reaction
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Receptors, N-Methyl-D-Aspartate / biosynthesis*
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Receptors, N-Methyl-D-Aspartate / physiology
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Recombinant Fusion Proteins / biosynthesis
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Scyphozoa
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Transfection / methods
Substances
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Ion Channels
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Luminescent Proteins
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Macromolecular Substances
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Receptors, N-Methyl-D-Aspartate
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Recombinant Fusion Proteins
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Green Fluorescent Proteins