Abstract
The E. coli AP1-200-9 strain for rapid identification of genes encoding restriction and modification enzymes carries a temperature sensitive lacZ gene fused to the damage-inducible dinD locus. A derivative of this strain was constructed that has a wild-type form of this locus which allows for a more efficient identification of recombinant plasmids encoding restriction and modifications enzymes.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / genetics
-
Cloning, Molecular
-
DNA Modification Methylases / genetics*
-
DNA Restriction Enzymes / genetics*
-
DNA, Recombinant / genetics
-
Escherichia coli / genetics*
-
Escherichia coli Proteins*
-
Lac Operon / genetics
-
Mutation
-
Phenotype
-
Plasmids / genetics*
-
SOS Response, Genetics
-
Temperature
Substances
-
Bacterial Proteins
-
DNA, Recombinant
-
Escherichia coli Proteins
-
dinD protein, E coli
-
DNA Modification Methylases
-
DNA Restriction Enzymes