Abstract
Six patients with previously diagnosed chronic myelogenous leukemia (CML) were studied by a tri-color immunophenotyping/FISH method for direct determination of the Philadelphia (Ph) chromosome in B and T lymphocytes. Two patients had involvement of CD20-positive lymphocytes. CD3-positive lymphocytes in all patients were negative for the Ph chromosome.
MeSH terms
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Antigens, CD / analysis
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Antigens, CD20
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Antigens, Differentiation, B-Lymphocyte / analysis
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CD3 Complex / analysis
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DNA Nucleotidylexotransferase / metabolism
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Humans
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Immunophenotyping
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In Situ Hybridization, Fluorescence / methods*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / diagnosis*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / immunology
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Lymphocytes / enzymology
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Peroxidase / metabolism
Substances
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Antigens, CD
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Antigens, CD20
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Antigens, Differentiation, B-Lymphocyte
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CD3 Complex
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Peroxidase
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DNA Nucleotidylexotransferase