Correlative retention time peak identification method for glycated haemoglobin in high-performance liquid chromatography

J Chromatogr A. 1994 Feb 11;661(1-2):143-51. doi: 10.1016/0021-9673(93)E0886-Y.

Abstract

A convenient peak identification method in stepwise elution was investigated and correlation among the retention times of peaks in ion-exchange chromatography of glycated haemoglobin was assessed. By using a correlation method, accuracy of peak identification among columns with degradation and product deviations can be maintained. The correlative retention time identification procedure is treated theoretically.

MeSH terms

  • Chromatography, High Pressure Liquid* / instrumentation
  • Chromatography, High Pressure Liquid* / methods
  • Chromatography, Ion Exchange
  • Fetal Hemoglobin / analysis
  • Glycated Hemoglobin / analysis*
  • Glycated Hemoglobin / chemistry
  • Humans
  • Time Factors

Substances

  • Glycated Hemoglobin A
  • Fetal Hemoglobin