The signal modulator protein 14-3-3 is a target of sphingosine- or N,N-dimethylsphingosine-dependent kinase in 3T3(A31) cells

Biochem Biophys Res Commun. 1995 Nov 22;216(3):739-47. doi: 10.1006/bbrc.1995.2684.

Abstract

Phosphorylation of a 28 kDa protein in 3T3(A31) cells upon addition of semi-purified kinase was induced only by sphingosine (Sph) or N,N-dimethylsphingosine (DMS), but not by 17 other lipids tested. The 28 kDa protein, a major target of Sph- or DMS-dependent kinase, is now identified as 14-3-3 protein, which modulates a number of key protein kinases involved in regulation of intracellular signaling (Aitken A, Trends Biochem Sci 20:95-97, 1995; Morrison D, Science 266:56-57, 1994). Thus, Sph and DMS modulate the function of a versatile modulator controlling a number of signaling molecules.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 14-3-3 Proteins
  • 3T3 Cells / enzymology*
  • Amino Acid Sequence
  • Animals
  • Enzyme Induction / drug effects
  • Enzyme Inhibitors / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Molecular Weight
  • Phosphorylation
  • Protein Kinases / metabolism*
  • Proteins / chemistry
  • Proteins / metabolism*
  • Signal Transduction
  • Sphingosine / analogs & derivatives*
  • Sphingosine / pharmacology*
  • Tyrosine 3-Monooxygenase*

Substances

  • 14-3-3 Proteins
  • Enzyme Inhibitors
  • Proteins
  • Tyrosine 3-Monooxygenase
  • Protein Kinases
  • N,N-dimethylsphingosine
  • Sphingosine