Characterization of the exposed carbohydrates on the surface membrane of adult Schistosoma mansoni by analysis of lectin binding

Parasitology. 1980 Aug;81(1):1-15. doi: 10.1017/s0031182000054986.

Abstract

The surface architecture of adult male Schistosoma mansoni was explored using a range of lectins with differing carbohydrate specificites. Highest specific binding was achieved with concanavalin A and the agglutinin of molecular weight 60000 from Ricinus communis; the binding of wheat germ agglutinin was mostly non-specific. Small amounts of peanut agglutinin and soybean agglutinin binding were observed and the binding of these lectins was increased by pre-treating the parasite with neuraminidase. The fucose binding protein of Lotus tetragonolobus failed to bind. These results indicate that mannose and/or glucose, galactose, N-acetylglucosamine, N-acetyl-galactosamine and sialic acid are exposed on the surface of the adult male schistosome.

MeSH terms

  • Acetylgalactosamine / analysis
  • Acetylglucosamine / analysis
  • Animals
  • Carbohydrates / analysis*
  • Cell Membrane / analysis
  • Concanavalin A / metabolism
  • Fucose / metabolism
  • Galactose / analysis
  • Glucose / analysis
  • Lectins / metabolism
  • Male
  • Mannose / analysis
  • Schistosoma mansoni / analysis*

Substances

  • Carbohydrates
  • Lectins
  • fucose-binding lectin
  • Concanavalin A
  • Fucose
  • Glucose
  • Acetylgalactosamine
  • Mannose
  • Acetylglucosamine
  • Galactose