Peroral immunization of germfree piglets: appearance of antibody-forming cells and antibodies of different isotypes

Scand J Immunol. 1981;13(5):467-72. doi: 10.1111/j.1365-3083.1981.tb00158.x.

Abstract

Precolostral germfree piglets were perorally immunized with sheep erythrocytes daily, beginning on the 5th day of life. At different intervals the numbers of IgM, IgG and IgA antibody-forming cells were estimated in the spleens, lymph nodes and intestinal mucosa by the haemolytic plaque method. In the beginning of the response IgM antibody-forming cells were detected in intestinal mucosa and mesenteric lymph nodes. IgA plaque-forming cells appeared later and were predominant during the whole period in all organs tested. In all lymphatic organs tested the appearance of IgG antibody-producing cells followed that of IgA plaque-forming cells. The development of immunoglobulins and antibody of different isotypes in intestinal contents and sera was compared with the cellular kinetics found in these young animals after local stimulation.

MeSH terms

  • Administration, Oral
  • Animals
  • Antibodies / classification*
  • Antibody-Producing Cells / immunology*
  • Germ-Free Life*
  • Immunization*
  • Immunoglobulin A
  • Immunoglobulin G
  • Intestinal Mucosa / immunology
  • Lymph Nodes / immunology
  • Protein Conformation
  • Spleen / immunology
  • Swine

Substances

  • Antibodies
  • Immunoglobulin A
  • Immunoglobulin G