[Effects of high density lipoproteins (HDL) and low density lipoproteins (LDL) on cultured bovine aorta endothelial cells. Preliminary observations on ultrastructural morphology of the plasma membrane by freeze-fracturing]

Boll Soc Ital Biol Sper. 1982 Nov 15;58(21):1387-93.
[Article in Italian]

Abstract

Bovine aortic endothelial cells (BAEC), detached with collagenase, have been studied morphologically after administration, in vitro, of human lipoprotein fractions (200 micrograms/ml of LDL, 200 micrograms/ml of HDL), 24 h before the confluence. One day after the treated cells and the control one were detached with trypsine and prepared for freeze-etching examination. The plasma-membrane has revealed, in the lipoprotein treated cells (HDL or LDL) an increased average value of the number of "pits" present upon a surface unit (P less than 0,05). Moreover, in the HDL-treated cells, the "pits", sometimes, have appeared with a different morphological evidence, similar to the aspects of exocytosis as seen e.g. in platelets or in Langerhans isle cells.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Aorta, Thoracic / cytology*
  • Aorta, Thoracic / drug effects
  • Cattle
  • Cell Membrane / ultrastructure
  • Cells, Cultured
  • Endothelium / ultrastructure
  • Freeze Fracturing
  • Lipoproteins, HDL / pharmacology*
  • Lipoproteins, LDL / pharmacology*
  • Microscopy, Electron

Substances

  • Lipoproteins, HDL
  • Lipoproteins, LDL