Enzyme-linked immunosorbent assay for detection of antibody to the hepatitis B surface antigen-associated delta antigen

J Clin Microbiol. 1981 Aug;14(2):173-7. doi: 10.1128/jcm.14.2.173-177.1981.

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) was developed for specific detection of antibody to the hepatitis B surface antigen-associated delta antigen. The sensitivity of ELISA was intermediate between that of previously described immunofluorescence and radioimmunological assays for anti-delta. Performance of ELISA was simple and required only ordinary and inexpensive laboratory equipment.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Viral / analysis*
  • Enzyme-Linked Immunosorbent Assay*
  • Fluorescent Antibody Technique
  • Hepatitis B Antibodies / analysis*
  • Hepatitis B Antigens / immunology*
  • Hepatitis B Surface Antigens / analysis
  • Hepatitis delta Antigens
  • Horseradish Peroxidase
  • Humans
  • Immunoenzyme Techniques*
  • Immunoglobulin G / analysis
  • Radioimmunoassay

Substances

  • Antibodies, Viral
  • Hepatitis B Antibodies
  • Hepatitis B Antigens
  • Hepatitis B Surface Antigens
  • Hepatitis delta Antigens
  • Immunoglobulin G
  • Horseradish Peroxidase