Abstract
The human cell line, GCT, secretes hemopoietins into serum-free culture medium. The conditioned medium contains activities that stimulate neutrophil-monocyte, macrophage, eosinophil, and erythroid colony growth in human marrow cultures. We have used hydrophobic adsorption chromatography to separate a neutrophil-monocyte colony-stimulating factor (CSF) from the other colony-stimulating activities. This hydrophobic CSF has no eosinophil-stimulating activity and is virtually devoid of erythroid-stimulating activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adsorption
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Animals
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Cattle
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Cell Line
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Chemical Fractionation
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Colony-Stimulating Factors / metabolism*
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Erythrocytes / metabolism*
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Humans
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Monocytes / metabolism*
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Sepharose / analogs & derivatives
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Sepharose / pharmacology
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Water / metabolism*
Substances
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Colony-Stimulating Factors
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Water
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phenyl-sepharose
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Sepharose