Flush exposure and injection immunization of rainbow trout to selected DNP conjugates

Dev Comp Immunol. 1983 Spring;7(2):261-8. doi: 10.1016/0145-305x(83)90007-1.

Abstract

Rainbow trout (Salmo gairdneri) were immunized by flush exposure or intraperitoneal injection with single doses of the following dinitrophenyl (DNP) conjugates: DNP-keyhole limpet hemocyanin (KLH), DNP-Ficoll, DNP-O-antigen (from the fish pathogen, Yersinia ruckeri), DNP-sheep red blood cells, or DNP-duck red blood cells. The immune response was demonstrated by the passive hemolytic plaque assay to show splenic antibody-producing cells (APC) 14 days after antigen administration. High numbers of splenic APC specific for the hapten were found when the conjugates DNP-KLH, DNP-Ficoll, and DNP-O-antigen were given by injection and when DNP-O-antigen was given by flush exposure. The heterologous red blood cells were relatively nonimmunogenic. When the immune response to the hapten and carrier could be measured--e.g., after immunization with the DNP-O-antigen--the numbers of APC were consistently higher for the hapten.

MeSH terms

  • Animals
  • Antibody Formation
  • Dinitrobenzenes / immunology
  • Erythrocytes / immunology
  • Fish Diseases / prevention & control
  • Haptens
  • Humans
  • Immunization
  • Salmonidae / immunology*
  • Trout / immunology*

Substances

  • Dinitrobenzenes
  • Haptens