Abstract
The glutamate dehydrogenase gene of Escherichia coli has been cloned into broad host-range plasmids and can complement glutamate synthase mutants of Methylophilus methylotrophus. Assimilation of ammonia via glutamate dehydrogenase is more energy-efficient than via glutamate synthase, thus the recombinant organism converts more growth substrate, methanol, into cellular carbon.
MeSH terms
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Adenosine Triphosphate / metabolism
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Ammonia / metabolism
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Bacterial Proteins / biosynthesis*
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DNA, Recombinant
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Escherichia coli / enzymology
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Genetic Engineering / methods
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Glutamate Dehydrogenase / genetics*
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Glutamate Synthase / metabolism
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Methanol / metabolism
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Methylococcaceae / enzymology
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Methylococcaceae / metabolism*
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Plasmids
Substances
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Bacterial Proteins
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DNA, Recombinant
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Ammonia
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Adenosine Triphosphate
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Glutamate Synthase
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Glutamate Dehydrogenase
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Methanol