The antibody response to a single antigenic determinant of the tobacco mosaic virus protein (TMVP): effects of allotype-linked genes and restricted heterogeneity of the response

J Immunol. 1983 Dec;131(6):2875-81.

Abstract

The antibody response to a decapeptide antigenic determinant representing residues 103 to 112 of the tobacco mosaic virus protein (TMVP) was studied with the synthetic decapeptide and a panel of its synthetic analogues. The anti-decapeptide response was found to be of restricted heterogeneity and was regulated by heavy chain allotype-linked (V region) genes on at least two levels. One level was whether or not a significant antibody response was made to the decapeptide determinant. Thus, strains with the Igh haplotypes j, o, e, and n were high responders, whereas strains with haplotype Ighb were low responders. The second level of V region gene control was with regard to the fine specificity of the anti-decapeptide antibodies produced. Using the peptide panel to analyze fine specificity, we define two new V region genetic markers, VH-TMVPj and VH-TMVPe.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Viral / biosynthesis*
  • Antibodies, Viral / genetics
  • Antibodies, Viral / immunology
  • Antibody Specificity
  • Binding Sites, Antibody
  • Genetic Linkage
  • Immunoglobulin Allotypes / genetics*
  • Immunoglobulin Variable Region / genetics*
  • Mice
  • Mice, Inbred A
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred NZB
  • Species Specificity
  • Tobacco Mosaic Virus / genetics
  • Tobacco Mosaic Virus / immunology*
  • Viral Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Immunoglobulin Allotypes
  • Immunoglobulin Variable Region
  • Viral Proteins