Non-radiochemical procedure for the measurement of O-methylation of the stereoisomers of isoprenaline

J Chromatogr. 1984 Oct 12;310(2):283-95. doi: 10.1016/0378-4347(84)80093-6.

Abstract

A non-radiochemical procedure has been developed which permits the separation and measurement of isoprenaline (ISO) and the O-methylated metabolite, 3-methoxyisoprenaline (MeOISO). This methodology employs alumina chromatography and toluene solvent extraction to separate the catecholamine, ISO, from the O-methylated derivative, MeOISO. High-performance liquid chromatography with electrochemical detection has been used to quantify these compounds. The biological application of this procedure includes the evaluation of O-methylation of the stereoisomers of ISO by intact tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Aorta, Thoracic / analysis
  • Catechol O-Methyltransferase Inhibitors
  • Catechols / analysis
  • Chromatography, High Pressure Liquid / methods
  • Electrochemistry
  • In Vitro Techniques
  • Isoproterenol / analysis*
  • Male
  • Methylation
  • Propiophenones / pharmacology
  • Rabbits
  • Stereoisomerism

Substances

  • Catechol O-Methyltransferase Inhibitors
  • Catechols
  • Propiophenones
  • U 0521
  • Isoproterenol