Polymorphism of sarcoplasmic-reticulum adenosine triphosphatase of rabbit skeletal muscle

Biochem J. 1981 Jul 1;197(1):245-8. doi: 10.1042/bj1970245.

Abstract

Antibody was raised in chickens against purified sarcoplasmic-reticulum Ca2+-activated ATPase (Ca2+-ATPase). The immunological relationship between the Ca2+-ATPase of fast-muscle and slow-muscle sarcoplasmic reticulum was investigated by a one-step and a two-step competitive enzyme-linked immunosorbent assay (ELISA). The results show marked antigenic differences between the membrane-bound Ca2+-ATPase of the sarcoplasmic-reticulum vesicles from fast muscle and slow muscle, beside differences in the membrane content of ATPase protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Calcium-Transporting ATPases / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Muscle Relaxation
  • Muscles / enzymology
  • Muscles / immunology*
  • Rabbits
  • Sarcoplasmic Reticulum / enzymology
  • Sarcoplasmic Reticulum / immunology*

Substances

  • Antibodies
  • Calcium-Transporting ATPases