A freezing method for cell fusions to distribute and reduce labor and permit more thorough early evaluation of hybridomas

J Immunol Methods. 1984 Jan 20;66(1):59-67. doi: 10.1016/0022-1759(84)90247-3.

Abstract

A method is described whereby cell fusions can be bulk-frozen shortly after the hybridization step. Recoveries are shown to be comparable to those obtained for control hybridomas cultured without freezing. Advantages are discussed in terms of labor distribution and antibody assay and evaluation strategies. It is further shown that peritoneal feeder cell preparations can be conveniently frozen as a means of workload reduction.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / standards
  • Ascitic Fluid / immunology
  • Cell Fusion
  • Freezing
  • Gonadotropin-Releasing Hormone / immunology
  • Hybridomas / immunology*
  • Immunologic Techniques* / economics
  • Mice
  • Mice, Inbred BALB C
  • Rats
  • Spleen / cytology

Substances

  • Antibodies, Monoclonal
  • Gonadotropin-Releasing Hormone