[Characterization of cell suspensions adhering to nylon obtained from mononuclear cells in toto or depleted of monocytes]

Boll Soc Ital Biol Sper. 1984 Dec 30;60(12):2317-23.
[Article in Italian]

Abstract

The aim of this work was to evaluate the effect of monocyte depletion on the preparation of B cell enriched suspensions for DR typing. Nylon fiber adherent-cells obtained from total, or monocyte depleted, MNC were identified using different surface markers and cytochemical staining (SIg, DR, ANAE). The results demonstrate that no significant improvement in B enrichment is obtained by monocyte depletion.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Surface / immunology*
  • B-Lymphocytes / immunology*
  • Cell Adhesion
  • Female
  • HLA Antigens*
  • Humans
  • In Vitro Techniques
  • Male
  • Monocytes / immunology*
  • Naphthol AS D Esterase
  • T-Lymphocytes / immunology

Substances

  • Antigens, Surface
  • HLA Antigens
  • Naphthol AS D Esterase