Cytotoxicity of anti-beta 2-microglobulin xenoantisera to human and murine myeloid progenitor cells and lack of cross-reactivity with colony-stimulating activity

J Immunol. 1980 Jun;124(6):2906-8.

Abstract

A previous report has suggested an antigenic relationship between beta 2-microglobulin (beta 2 mu) and granulocyte colony-stimulating activity (CSA). Since human myeloid progenitor cells (CFU-C) express HLA antigens and beta 2 mu is a known molecular component of HLA antigens, we wondered whether the reported effect of anti-beta 2 mu heteroantisera on in vitro granulopoiesis might result from cytotoxicity to CFU-C rather than from cross-reactivity with CSA. To test this, we used rabbit antibody reactive with human and murine beta 2 mu (anti-beta 2 mu). Treatment of human and murine bone marrow cells with anti-beta 2 mu and complement resulted in 95+% inhibition of CFU-C colony formation compared to controls. To test for an effect on CSA, anti-beta 2 mu was incubated with human and murine sources of CSA. After addition of goat anti-rabbit Ig antiserum to precipitate immune complexes and unbound anti-beta 2 mu, the supernatant fluid retained CSA but was no longer cytotoxic to CFU-C. These results indicate that human and murine CFU-C express membrane beta 2 mu and that anti-beta 2 mu antibody does not cross-react with human or murine CSA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Beta-Globulins / immunology*
  • Bone Marrow Cells*
  • Colony-Forming Units Assay
  • Colony-Stimulating Factors / pharmacology
  • Cross Reactions
  • Cytotoxicity, Immunologic*
  • Humans
  • Immune Sera / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • beta 2-Microglobulin / immunology*

Substances

  • Beta-Globulins
  • Colony-Stimulating Factors
  • Immune Sera
  • beta 2-Microglobulin