Purification of single- and double-length M13 virions by polyacrylamide gel electrophoresis

J Virol. 1974 Feb;13(2):470-7. doi: 10.1128/JVI.13.2.470-477.1974.

Abstract

Complete separation of single- and double-length M13 virions from each other and from still-longer phage particles was achieved by electrophoresis of phage stocks through 2.5% polyacrylamide gels.

MeSH terms

  • Coliphages / growth & development
  • Coliphages / isolation & purification*
  • Crosses, Genetic
  • DNA, Viral
  • Diploidy
  • Electrophoresis, Polyacrylamide Gel*
  • Escherichia coli
  • Haploidy
  • Heterozygote
  • Methods
  • Microscopy, Electron
  • Mutation
  • Polyploidy
  • Thymidine
  • Tritium

Substances

  • DNA, Viral
  • Tritium
  • Thymidine