Antibodies to Z DNA stabilized with polyarginine

FEBS Lett. 1985 Oct 21;191(1):67-71. doi: 10.1016/0014-5793(85)80995-9.

Abstract

The left-handed form of poly(dG-m5dC).poly(dG-m5dC) induced by heating the copolymer in the presence of magnesium and stabilized with polyarginine can be used to raise antibodies in rabbits. These antibodies are able to recognize the Z conformation of both methylated and nonmethylated forms of the copolymers. In the same experimental conditions, hypermethylated B DNA is not recognized by these antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology*
  • Circular Dichroism
  • DNA / immunology*
  • Filtration
  • Methylation
  • Nucleic Acid Conformation
  • Peptides / pharmacology*
  • Rabbits
  • Temperature

Substances

  • Antibodies
  • Peptides
  • polyarginine
  • DNA