Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer

Nature. 1985 Aug;316(6029):606-9. doi: 10.1038/316606a0.

Abstract

We have introduced a full-length complementary DNA of the T-cell antigen receptor beta-chain into a mutant human T-cell line that lacked a complete beta-chain messenger RNA, had a diminished level of alpha-chain transcript and did not express surface T3- or antigen receptor. Expression of the transfected beta-chain led to a normal level of alpha-chain transcript and a structurally and functionally active T3 T-cell antigen receptor complex on the cell surface.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • DNA / genetics*
  • Humans
  • Mutation
  • Proteins / analysis
  • RNA, Messenger / analysis
  • Receptors, Antigen, T-Cell / analysis*
  • Receptors, Antigen, T-Cell / genetics
  • Transcription, Genetic
  • Transformation, Genetic*

Substances

  • Proteins
  • RNA, Messenger
  • Receptors, Antigen, T-Cell
  • DNA