In a natural focus of paragonimiasis in the northeastern region of Venezuela, 130 people were clinically, parasitologically and immunologically evaluated. A specific intradermal test was positive in 13%. Besides an index case, three other active cases of paragonimiasis were identified. Adult Paragonimus worms and metacercariae were recovered from Didelphis marsupialis and Eudaniela garmani, respectively. Previous reports of Paragonimus infection in Venezuela are discussed.