Comprehensive Analysis of Metabolic Changes in Mice Exposed to Corilagin Based on GC-MS Analysis

Drug Des Devel Ther. 2025 Jan 21:19:389-404. doi: 10.2147/DDDT.S482510. eCollection 2025.

Abstract

Background: Corilagin is widely distributed in various medicinal plants. In recent years, numerous pharmacological activities of Corilagin have been reported, including anti-inflammatory, antiviral, hepatoprotective, anti-tumor, and anti-fibrosis effects. However, there is still a need for systematic metabolomics analysis to further elucidate its mechanisms of action. The aim of this study was to explore the pharmacological mechanism of Corilagin.

Methods: This study utilized gas chromatography-mass spectrometry (GC-MS) to analyze central target tissues, comprehensively exploring the pharmacological mechanism of Corilagin in mouse models. We identified the differential metabolites by multivariate analyses, which include principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Using MetaboAnalyst 5.0 and the KEGG database was used to depict the 12 key metabolic pathways.

Results: Compared with the control group, the Corilagin induced 20, 9, 11, 7, 16, 19, 14, 15, and 16 differential metabolites in the intestine, lung, kidney, stomach, heart, liver, hippocampus, cerebral cortex, and serum, respectively. And 12 key pathways involving glucose metabolism, lipid metabolism, and amino acid metabolism were identified following Corilagin treatment.

Conclusion: This research provides insight into the action mechanism of Corilagin's anti-oxidative, anti-inflammatory, anti-atherosclerotic, hepatoprotective, anti-tumor, and neuroprotective properties.

Keywords: Corilagin; amino acids; gas chromatography-mass spectrometry; metabolomics; pharmacological mechanism.

MeSH terms

  • Animals
  • Gas Chromatography-Mass Spectrometry*
  • Glucosides* / pharmacology
  • Hydrolyzable Tannins* / administration & dosage
  • Hydrolyzable Tannins* / pharmacology
  • Male
  • Metabolomics
  • Mice

Substances

  • Hydrolyzable Tannins
  • corilagin
  • Glucosides

Grants and funding

This study was supported by the grants from National Natural Science Foundation of China (No. 82302795), Natural Science Foundation of Shandong Province (No. ZR2023MH365, ZR2023QH094), Key Research and Development Projects of Jining City (No. 2022YXNS019). Traditional Chinese Medicine Science and Technology Project of Shandong province (No. Q-2023058). High-level Training Program of Jining Medical University (JYGC2021FKJ009).