The evaluation of nanoplastics bioaccumulation in living organisms is still considered an emerging challenge, especially as global plastic production continues to grow, posing a significant threat to humans, animals, and the environment. The goal of this work is to advance the development of standardized methods for reliable biomonitoring in the future. It is crucial to employ sensitive techniques that can detect and measure nanoplastics effectively, while ensuring minimal impact on the environment. To understand nanoplastics retention by freshwater organisms, phyto- and zooplankton, and mussels were exposed to gold-doped polymeric nanoparticles synthesized in our laboratory. The results demonstrated that measuring gold content using inductively coupled plasma mass spectrometry (ICP-MS), along with confirmation of its presence through electron microscopy in selected exposed samples provides insight into the accumulation and release of nanoplastics by organisms playing a relevant ecological role at the early levels of aquatic food webs.
Keywords: ICP-MS; bioaccumulation; electron microscopy; freshwater organisms; gold nanoparticles; nanoplastics.